Title: Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A). | Journal: FEBS letters. 2012 Apr;586(8):1079-85 | Authors: Wu F, Wang S, Xing J, Li M, Zheng C. | Abstract: WD repeat-containing protein 42A (WDR42A) is a member of the WD40-repeat proteins. Here, we investigated the localization pattern of WDR42A in living cells. By mutational analysis, a nuclear localization signal, 114PRRRVQRKR122, was for the first time determined. The dominant negative, co-immunoprecipitation and GST pull-down results further demonstrated that the nuclear import of WDR42A was mediated by karyopherin-α1/β1 in conjunction with the GTPase Ran. Additionally, a nuclear export signal, 39IEVEASDLSLSL50, was verified to be a functional NES, which mediated the nuclear export through Chromosome Region Maintenance 1 dependent pathway. All these data suggest WDR42A is a nucleocytoplasmic shuttling protein. | See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/22500989 |
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