Title: Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature. |
Journal: Journal of bacteriology. 1989 Mar;171(3):1742-3 |
Authors: Ito K, Hirota Y, Akiyama Y. |
Abstract: Phenotypes of secY and secA temperature-sensitive mutants at permissive (low) temperature have been examined. The secY24 mutant was found to be extremely susceptible to export inhibition by a basal-level synthesis of the MalE-LacZ 72-47 hybrid protein or to overproduction of a normal secretory protein such as maltose-binding protein or beta-lactamase. Comparison of this phenotype of secY24 with those of the secY100 and secA51 mutants under similar conditions suggested that MalE-LacZ protein and overproduced secretory protein do not nonspecifically enhance the partial secretion defect but act synergistically with secY24 to inhibit protein export. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/2646297 |