Title: Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. |
Journal: Proteins. 1997 Jun;28(2):298-300 |
Authors: Tian Y, Beaman TW, Roderick SL. |
Abstract: A chloramphenicol acetyltransferase from Pseudomonas aeruginosa genomic DNA has been overexpressed, refolded, purified, and crystallized. Crystals suitable for a three-dimensional x-ray structure determination were obtained from solutions of polyethyleneglycol methyl ether 2000 containing NiCl2 at pH 8.5. These crystals belong to the cubic space group P4(1/3)32 (a = 154.8 A) and diffract x-rays to approximately 3.2 A resolution. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/9188747 |