Title: Genome organization of membrane-containing bacteriophage PRD1. | Journal: Virology. 1991 Aug;183(2):658-76 | Authors: Bamford JK, Hänninen AL, Pakula TM, Ojala PM, Kalkkinen N, Frilander M, Bamford DH. | Abstract: We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome. | See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/1853567 |
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