Title: The role of SmpB protein in trans-translation. |
Journal: FEBS letters. 2002 Mar;514(1):74-7 |
Authors: Shimizu Y, Ueda T. |
Abstract: The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/11904185 |