Title: DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. | Journal: Nucleic acids research. 1987 Jan;15(2):771-84 | Authors: Adachi T, Mizuuchi M, Robinson EA, Appella E, O'Dea MH, Gellert M, Mizuuchi K. | Abstract: We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene. | See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/3029692 |
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