Title: DNA gyrase: an enzyme that introduces superhelical turns into DNA. |
Journal: Proceedings of the National Academy of Sciences of the United States of America. 1976 Nov;73(11):3872-6 |
Authors: Gellert M, Mizuuchi K, O'Dea MH, Nash HA. |
Abstract: Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/186775 |