Title: Transcriptional analysis of the pst operon of Escherichia coli. | Journal: Molecular genetics and genomics : MGG. 2002 Dec;268(4):518-24 | Authors: Aguena M, Yagil E, Spira B. | Abstract: The pst operon of Escherichia coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation. A phosphate-regulated promoter located upstream of the most proximal gene ( pstS) controls the transcription of the entire operon. Though the full-length pst mRNA could be detected by an improved RT-PCR protocol, Northern analysis using several pst-specific probes failed to reveal this transcript. Instead, smaller but distinct pst mRNA species were evident. Primer-extension experiments localized the 5' ends of pst mRNAs within the operon. The data suggest that the full-length mRNA is rapidly processed post-transcriptionally. | See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/12471449 |
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