Title: The TolC protein of Escherichia coli K12 is synthesised in a precursor form. |
Journal: FEBS letters. 1983 Jun;156(2):307-10 |
Authors: Hackett J, Misra R, Reeves P. |
Abstract: We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse-chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS-PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precursor form (Mr 54 500) which could be chased into the mature form (Mr 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N-terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (Mr 2542). The initiator Met was preceded by a Shine-Dalgarno sequence, with the correct spacing. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/6303857 |