Title: Nucleotide sequence and expression of the beta-lactamase gene from Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. |
Journal: Journal of bacteriology. 1987 Apr;169(4):1763-6 |
Authors: Wang PZ, Novick RP. |
Abstract: The structural gene for beta-lactamase in the Staphylococcus aureus plasmid pI258 was cloned into a Staphylococcus aureus-Bacillus subtilis-Escherichia coli shuttle vector, pWN101, and the nucleotide sequence of the gene was determined. pWN101 was structurally stable and the beta-lactamase gene was expressed efficiently from its native promoter and ribosome-binding site in all three hosts. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/3104315 |