Title: Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes. | Journal: Journal of bacteriology. 2001 Apr;183(8):2700-3 | Authors: Galvani C, Terry J, Ishiguro EE. | Abstract: The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation. | See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/11274135 |
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