Title: The path of messenger RNA through the ribosome. | Journal: Cell. 2001 Jul;106(2):233-41 | Authors: Yusupova GZ, Yusupov MM, Cate JH, Noller HF. | Abstract: Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting. | See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/11511350 |
|