Title: Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor. |
Journal: Proceedings of the National Academy of Sciences of the United States of America. 1983 Aug;80(15):4659-63 |
Authors: Abdel-Monem M, Taucher-Scholz G, Klinkert MQ. |
Abstract: Active DNA helicase I (Mr 180,000) can be isolated from Escherichia coli F+ strains but not F- strains. The transfer of the F sex factor to F- strains by conjugation permits the purification of the enzyme from the transconjugant strains. We conclude from this that helicase I is coded for by a portion of the F factor. Results also obtained by using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggest that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor. |
See full PubMed entry: http://www.ncbi.nlm.nih.gov/pubmed/6308637 |